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KMID : 0364920080330030087
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Journal of Radiation Protection and Research
2008 Volume.33 No. 3 p.87 ~ p.91
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The Effect of Bu-Zhong-Yi-Qi-Tang on Epidermal Melanocytes in Ultraviolet B-irradiated Mice
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Lee Hae-June
Kim Hwan-Sung
Park Young-Jong
Kim Joong-Sun
Kim Jong-Choon
Bae Chun-Sik
Jo Sung-Kee
Kim Seong-Ho
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Abstract
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We induced the activation of melanocytes in the epidermis of C57BL/6 mice by ultraviolet B (UVB) irradiation and observed the effect of Bu-Zhong-Yi-Qi-Tang (BZYQT) on the formation, and decrease of UVB-induced epidermal melanocytes.
C57BL/6 mice were irradiated by UVB 80 mJ/cm2 (0.5 mW/sec) daily for 7 days, and BZYQT was intraperitoneally or topically applied pre- or post-irradiation. For the estimation of change of epidermal melanocytes, light microscopic observation with dihydroxyphenylalanine (DOPA) stain was performed. Split epidermal sheets prepared from the ear of untreated mice exhibited 11-16 melanocytes/mm2, and one week after UV irradiation, the applied areas show an increased number of strongly DOPA-positive melanocytes with stout dendrites. But intraperitoneal or topical treatment with BZYQT before each irradiation interrupted UVBinduced pigmentation and resulted in a marked reduction in the number of epidermal melanocytes as compared to radiation control skin. The number and size of DOPA-positive epidermal melanocytes were also significantly decreased in intraperitoneally injected or topically applicated group after irradiation with BZYQT at 3rd and 6th weeks after irradiation. The present study suggests the BZYQT as inhibitor of UVB-induced pigmentation and depigmenting agent.
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KEYWORD
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Ultraviolet B, Melanocyte, Bu-Zhong-Yi-Qi-Tang
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